One-Step qPCR-realtime™ SARS CoV-2 E-CY5 gene
Multiplex OneqPCR- Raltime TM SARS CoV-2 (E) amplifies and detect the E gene (SARS CoV-2) and RP gene (Rnase P human) in Single One Step RT-PCR Reaction.
The kit contain Primer & Probe Mix for the detection of E gene (CY5) and Rp gene (HEX)
Positive Control for the amplification of the E gene is also included.
The One qPCR Enzyme Mix includes Reverse Transcriptase, Recombinant Ribonuclease Inhibitor in an optimized formulation, they also include Bioingentech® Taq DNA polymerase and all reagents for an optimized RT-qPCR.
The SARS CoV-2 (E-gene) primer and probe mix provided in the kit hybridize in specific and conserved zones of the E gene which generate an envelope protein which plays a central role in virus morphogenesis and assembly. This gen can be detected through your real time platform by the 5’ nuclease PCR detection method. During PCR amplification, forward and reverse primers hybridize to the cDNA generated SARS CoV-2 target. Fluorogenic probes are included in the same reaction mixture. The E gene is amplified and detected in the Cy5 channel.
An additional primer/probe set to detect the human RNase P gene (RP) as internal control to monitor PCR inhibition is also included in the panel labeled with a 5-reporter and a 3-quencher. During PCR amplification, the probe is cleaved and the reporter dye and quencher are separated. As a result, a fluorescence increase can be detected on a range of real time PCR platforms through HEX/VIC channel.
Figure 1: Clinical tests of the One-Step qPCR-realtime™ SARS CoV-2 E-CY5 detection Kit were performed with more than 5k samples from asymptomatic and symptomatic patients.
Total RNA was isolated followed by a One Step RT-PCR for the detection of the SARS CoV-2 E gene and the human RNase P gene.