E. coli O101:H4

Escherichia coli O101:H4 is a serotype of E. coli, a Gram-negative bacterium of the intestinal tract of animals and humans. The O101 serogroup has been associated with enterotoxigenic E. coli (ETEC) strains that cause neonatal diarrhoea in calves and piglets. Serotype-specific detection supports epidemiological surveillance and the characterisation of E. coli isolates from livestock.

The OneqPCR-realtime™ E. coli O101:H4 Kit is a ready-to-use assay for the specific detection of E. coli O101:H4 DNA by real-time PCR (qPCR). Specific primers and a FAM-labelled hydrolysis probe amplify the target, while an internal control detected in the HEX channel reveals possible PCR inhibition or extraction failure. Positive and negative controls are included, and results can be analysed qualitatively or quantitatively using a standard curve.