Avian influenza virus serotype N2

Avian influenza viruses are type A influenza viruses classified by their surface haemagglutinin (H) and neuraminidase (N) proteins. The N2 neuraminidase subtype is present in several relevant avian strains, such as H9N2, which is endemic in poultry in Asia, the Middle East and Africa, as well as some H5N2 and H7N2 viruses. Identifying the N subtype is essential to characterise avian influenza outbreaks and for surveillance of strains with zoonotic potential.

The OneqPCR-realtime™ Avian influenza virus serotype N2 Kit is a ready-to-use assay for the specific detection of Avian influenza virus serotype N2 RNA by one-step real-time RT-PCR (qRT-PCR): reverse transcription and amplification take place in a single tube. Specific primers and a FAM-labelled hydrolysis probe amplify the target, while an internal control detected in the HEX channel reveals possible PCR inhibition or extraction failure. Positive and negative controls are included, and results can be analysed qualitatively or quantitatively using a standard curve.