At SITC 2025, Lunaphore Technologies and ACD (a Bio-Techne brand) are showcasing a fully integrated spatial biology workflow that combines high-plex RNA and protein detection on the same tissue section. The COMET™ platform together with the SPYRE™ portfolio offers a powerful, automated solution for discovery and translational research in immuno-oncology and beyond.
Multiomics with COMET™ and RNAscope™ HiPlex Pro
Lunaphore’s COMET™ platform now brings together its seqIF™ protein detection technology with ACD’s RNAscope™ HiPlex Pro assay in a single automated workflow. This integration enables:
- Simultaneous detection of RNA and protein targets on the same tissue section.
- Automated, walk-away operation from staining to imaging.
- A true end-to-end spatial biology pipeline, from discovery studies to translational applications.
By combining highly sensitive RNA in situ hybridization with multiplex immunofluorescence, researchers can obtain richer spatial context, better understand cell states and interactions, and accelerate biomarker discovery in complex tissues.
SPYRE™ Portfolio: Amplifying Signals and Expanding Panels
The SPYRE™ portfolio is designed to complement COMET™ and help researchers tackle low-expressed and challenging biomarkers, while simplifying panel design.
SPYRE™ Amplification Kit – Amplify Low-Expressed Markers
Not all protein markers are easily detected with standard indirect immunofluorescence. Low-abundance or weakly expressed targets can be especially difficult to visualize.
The SPYRE™ Amplification Kit addresses this challenge by:
- Boosting the signal of low-expressed or hard-to-detect markers.
- Leveraging Lunaphore’s seqIF™ technology for high-sensitivity detection.
- Enabling more confident interpretation of subtle or rare targets within the tissue microenvironment.
This allows researchers to push their multiplex panels further without compromising sensitivity.
SPYRE™ Focus Panels – Tumor, Stroma and Vessel Biology
The expanding SPYRE™ Focus Panels are curated, modular antibody panels that target key biological domains:
- SPYRE™ Stroma Focus Panel – for interrogating stromal components and their role in the tumor microenvironment.
- SPYRE™ Vessel Focus Panel – for analyzing vascular markers and angiogenesis-related processes.
- Individual melanoma tumor markers – for more precise profiling in metastatic melanoma and other tumor contexts.
These validated panels:
- Streamline experimental design by reducing the need for individual antibody optimization.
- Accelerate biomarker discovery, helping labs quickly generate comprehensive spatial profiles.
- Cover tumor, stromal, and vascular compartments, providing a more complete view of tissue architecture and cell–cell interactions.
Meet Lunaphore at SITC 2025
At SITC 2025, Lunaphore is welcoming visitors at booth #603. During the conference, attendees will be able to:
- Get a personalized demo of the COMET™ platform and explore raw multiomic and proteomic tissue data, including the latest HORIZON™ software features.
- Book 1:1 meetings with Lunaphore leadership or product specialists to discuss implementation, workflow integration and specific research questions.
- Discover an innovative protease-free portfolio powered by RNAscope™ technology, designed to preserve tissue morphology while enabling high-quality data from discovery through translational research.
- Learn about new additions to the COMET™ and SPYRE™ portfolios.
- Explore novel data releases in immuno-oncology, including insights from the SECOMBIT clinical trial on metastatic melanoma.
Want to Learn More?
If your lab is exploring spatial biology, multiomics or high-plex tissue imaging, the combination of COMET™, RNAscope™ HiPlex Pro and SPYRE™ offers a robust, scalable solution for both discovery and translational workflows.
You can:
- Visit Lunaphore at SITC 2025, booth #603.
- Reach out to us for more information on how these technologies can complement your molecular diagnostics and research workflows.