Advancing Veterinary Molecular Diagnostics
At BioinGentech, our scientific team continues to strengthen the reliability and traceability of molecular diagnostics for animal health.
We’re proud to announce the completion of the validation report for the OneqPCR-realtime™ Bluetongue Virus kit, a diagnostic assay designed for rapid and sensitive detection of all 29 recognized serotypes of Bluetongue Virus (BTV).
About Bluetongue Disease
Bluetongue Virus is an arthropod-borne virus transmitted mainly by Culicoides midges, affecting sheep, cattle, and goats.
The disease causes fever, edema, mucosal lesions, and, in severe cases, high mortality in susceptible breeds. Beyond its clinical impact, Bluetongue outbreaks can disrupt international trade, reduce livestock productivity, and create substantial economic losses for the agricultural sector.
About the OneqPCR-realtime™ BTV Kit
The OneqPCR-realtime™ Bluetongue Virus assay targets segment 10 of the viral genome, enabling broad-range molecular detection across all serotypes.
This approach ensures early and accurate identification, supporting epidemiological surveillance, outbreak control, and veterinary research worldwide.
Validation Summary
Our validation process confirmed the kit’s accuracy, reliability, and robustness under diverse conditions:
- High Specificity: Detects Bluetongue Virus (BTV-1 to BTV-29) without cross-reaction with other viruses such as EHDV, Akabane, Schmallenberg, or FMDV.
- Instrument Compatibility: Validated on 25+ PCR platforms, including Bio-Rad CFX, ABI QuantStudio, Qiagen Rotor-Gene, and Roche LightCycler systems.
- Limit of Detection (LOD): Detects as few as 100 RNA copies/μL, ensuring sensitivity for early diagnosis.
- Robustness and Stability: Maintains consistent performance up to 2 years after opening when stored under recommended conditions.
- Validated Sample Matrices: Blood, tissue, swabs, and semen samples from ruminant species.
Supporting Global Animal Health
This validation reinforces BioinGentech’s commitment to developing scientifically validated molecular tools that enhance disease monitoring, trade security, and animal welfare.
Our OneqPCR-realtime™ Bluetongue Virus kit stands as a trusted choice for veterinary laboratories worldwide.
Our mission
Beyond its laboratory performance, this validation underscores our mission to bridge science and global health.
Each verified diagnostic tool contributes to faster outbreak response, safer trade of animal products, and sustainable livestock management — helping veterinary communities worldwide safeguard animal and public health.
Learn more
Please, download and read more about our Bluetongue PCR Kit: