Lymphocystis disease virus (LCDV) is a large (200–300nm in diameter) DNA virus which belongs to the genus which belongs to the genus lymphocystivirus in the family Iridoviridae, is the causative agent of lymphocystis disease characterized by formation of hypertrophied cells on fish skin, mouth, and fins, and has affected more than 140 marine and freshwater fish species worldwide, resulting in great economic losses. Viral isolation of LCDV is difficult and time-consuming; the development of a rapid and specific tool to detect LCDV is desirable for both diagnosis and pathogenic studies.
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