Burkholderia spp.

Burkholderia is a genus of Gram-negative, rod-shaped bacteria widely distributed in soil and water. It includes major animal and human pathogens such as B. mallei, the agent of glanders in horses, and B. pseudomallei, the cause of melioidosis, as well as members of the B. cepacia complex, which are opportunistic pathogens in immunocompromised hosts. Their intrinsic resistance to many antibiotics makes rapid molecular identification especially valuable.

The OneqPCR-realtime™ Burkholderia spp. Kit is a ready-to-use assay for the specific detection of Burkholderia spp. DNA by real-time PCR (qPCR). Specific primers and a FAM-labelled hydrolysis probe amplify the target, while an internal control detected in the HEX channel reveals possible PCR inhibition or extraction failure. Positive and negative controls are included, and results can be analysed qualitatively or quantitatively using a standard curve.