Maximising the accuracy of PCR tests is essential for companies that rely on accurate diagnostics. Here are some practical tips to ensure optimal results, whether your laboratory is involved in veterinary diagnostics, food safety or water analysis.
1. Proper sample collection and storage
Contamination during the sampling phase can lead to unreliable results. Always use sterile tools and ensure that samples are stored at the recommended temperatures.
2. Maintain equipment calibration
Regularly calibrate thermocyclers and other laboratory equipment. Small deviations in temperature can significantly affect the quality of amplification.
3. Follow the Kit protocols
Each PCR kit comes with specific instructions. Deviating from them can lead to inefficiencies or erroneous results. Always refer to the manufacturer’s guidelines for setup and thermocycling parameters.
4. Optimise Reaction Conditions
- Ensure accurate pipetting to avoid reagent imbalances.
- Run control samples to validate the performance of the assay.
- Use high-quality reagents and primers.
The application of these best practices not only improves test accuracy, but also minimises costly errors, improving the efficiency and reputation of the laboratory.
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